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Optimization of glutamate concentration and pH for H 2 production from volatile fatty acids by Rhodopseudomonas capsulata
Author(s) -
Shi X.Y.,
Yu H.Q.
Publication year - 2005
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2005.01700.x
Subject(s) - propionate , butyrate , rhodopseudomonas palustris , yield (engineering) , chemistry , rhodospirillales , response surface methodology , glutamate receptor , biochemistry , bacteria , rhodospirillaceae , nuclear chemistry , chromatography , photosynthesis , biology , fermentation , materials science , receptor , metallurgy , genetics
Aims: This study attempted to employ response surface methodology (RSM) to evaluate the effects of glutamate concentration and pH on H 2 production from volatile fatty acids by Rhodopseudomonas capsulata . Methods and Results: A mixture of acetate, propionate and butyrate was used as a carbon source for the H 2 production by R. capsulata . The H 2 yield and H 2 production rate were strongly affected by the glutamate concentration, pH and their interaction. The predicted maximum H 2 yield of 0·534 was obtained when glutamate concentration and pH were 6·56 mmol l −1 and 7·29 respectively. On the contrary, the maximum H 2 production rate of 18·72 ml l −1 h −1 was achieved at a glutamate concentration of 7·01 mmol l −1 and pH 7·31. Conclusions: Taking H 2 yield and H 2 production rate together into account, a glutamate concentration of 6·56–7·01 mmol l −1 and pH of 7·29–7·31 should be selected for H 2 production from a mixture of acetate, propionate and butyrate by R. capsulata . Significance and Impact of the Study: The RSM was a useful tool for maximizing H 2 production by photosynthetic bacteria (PSB).