Premium
Screening of bacterial associates of marine sponges for single cell oil and PUFA
Author(s) -
Patnayak S.,
Sree A.
Publication year - 2005
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2005.01671.x
Subject(s) - polyunsaturated fatty acid , eicosapentaenoic acid , bacillus subtilis , food science , fatty acid , biology , bacteria , incubation , biochemistry , bacterial cell structure , sudan black b , chemistry , microbiology and biotechnology , staining , genetics
Aim: To screen bacterial associates from marine sponges for single cell oil (SCO)/polyunsaturated fatty acid (PUFA) production. Methods and Results: Using Sudan black ‘B’ staining technique the bacterial associates were screened for cellular lipid accumulation, effect of culture media, incubation period and C : N ratio. Extraction of the bacterial lipids was carried out by Floch's method and fatty acid methyl esters were analysed by GC and GC/MS. Four bacterial associates of 50 isolated from eight marine sponges tested positive for lipid accumulation. Two bacterial associates, viz. Bacillus subtilis (RRL‐8) from Aurora globostellata and Pseudomonas spp. (RRL‐28) from Heteronema erecta were found to produce total lipids 16·9 and 31·7%, respectively, of their dry biomass. Conclusions: Increase in C : N ratio significantly improved lipid production to 33·4 and 42·7%. Both the isolates produced γ ‐linolenic acid (18 : 3 ω 6; 4·5 and 1·12% respectively), whereas B. subtilis showed 3·8% of eicosapentaenoic acid (20 : 5 ω 3) along with branched chain fatty acids. Significance and Impact of the Study: This is the first report of oleaginous bacterial associates from marine sponges.