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Controlling production of brominated cyclic depsipeptides by Pseudoalteromonas maricaloris KMM 636 T
Author(s) -
Sobolevskaya M.P.,
Smetanina O.F.,
Speitling M.,
Shevchenko L.S.,
Dmitrenok P.S.,
Laatsch H.,
Kuznetsova T.A.,
Ivanova E.P.,
Elyakov G.B.
Publication year - 2005
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2005.01635.x
Subject(s) - depsipeptide , chemistry , pseudoalteromonas , nutrient , mass spectrometry , in vitro , biochemistry , chromatography , organic chemistry , gene , 16s ribosomal rna
Aims: This study aims at evaluating the impact of the nutrient medium components on the in vitro production of the cytotoxic alterochromides. Methods and Results: The employment matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) facilitated the identification of a range of brominated cyclic depsipeptides with molecular masses of 843/845, 857/859 and 922/924/926 Da, and 936/938/940 Da produced by the marine bacterium Pseudoalteromonas maricaloris KMM 636 T . The fractions of cytotoxic yellow pigments yielded after methanol extraction of P. maricaloris KMM 636 T cells grown on five nutrient media were solely composed of brominated cyclic depsipeptides. Bromo‐alterochromides A and B were obtained after cultivation on low nutrient media, while dibrominated derivatives were the principal components of the biosynthesis during cultivation on nutrient rich media. Conclusions: The quantity of bromo‐alterochromides and their dibromo‐ derivates varied depending on the media composition. Significance and Impact of the Study: MALDI‐TOF mass spectrometry enables to generate accurate mass analysis for the identification of peptide and its derivates which is important in controlling the production of biologically active compounds in vitro .