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Performance of a new chromogenic plating medium for the isolation of Listeria monocytogenes from marine environments
Author(s) -
El Marrakchi A.,
Boum'handi N.,
Hamama A.
Publication year - 2005
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2004.01651.x
Subject(s) - chromogenic , listeria monocytogenes , isolation (microbiology) , plating (geology) , microbiology and biotechnology , food science , biology , chromatography , chemistry , bacteria , paleontology , genetics
Aims: This study investigated the performance of a new chromogenic plating medium for the detection of Listeria monocytogenes from naturally contaminated samples obtained from marine environments in Morocco in comparison with the conventional plating media PALCAM and Oxford. Methods: A total of 479 marine samples (sea water, sediment and mussels) were collected from 16 littoral sites in the region of Agadir (western centre of Morocco). They were examined for the presence of L. monocytogenes using a slight modification of the standardized French method (AFNOR V 08‐055) for the detection of L. monocytogenes from food and three different isolation media: PALCAM, Oxford and a new chromogenic plating medium. Results and Significance of the Study: The Oxford and the new chromogenic plating media were found relatively more efficient than the PALCAM medium for the isolation of L. monocytogenes (chi‐square test, P < 0·05) from marine samples. However, the new chromogenic plating medium was significantly more selective for L. monocytogenes ( P < 0·005) than the two other isolation media as 87·5% of the suspect colonies on this medium were indeed confirmed through identification of the isolates vs 12·7% for Oxford and only 3·8% for the PALCAM medium.