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Effect of human milk on type 1 and P‐fimbrial mRNA expression in intestinal Escherichia coli strains
Author(s) -
Nowrouzian F.L.,
Monstein H.J.,
Wold A.E.,
Adlerberth I.
Publication year - 2005
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2004.01631.x
Subject(s) - fimbria , escherichia coli , bacterial adhesin , microbiology and biotechnology , biology , operon , enterobacteriaceae , agar , messenger rna , gene , bacteria , biochemistry , genetics
Aims: Escherichia coli from breastfed infants express more type 1 fimbriae and less P fimbriae than E. coli from bottle‐fed infants. In this study we investigated the effect of human milk on production of mRNA for fimA (type 1 fimbriae) and papC (P fimbriae) in E. coli . Methods and Results: Production of adhesin gene mRNA was estimated using a reverse transcriptase polymerase chain reaction in E. coli strains under different culture conditions. More type 1 fimbrial mRNA was produced after culture in human milk ( P = 0·001) or Luria broth ( P = 0·014) than after culture on agar, whereas P‐fimbrial mRNA production was similar under all tested growth conditions. When cultured on agar, E. coli strains carrying both the fim and pap operons produced less type 1 and P‐fimbrial mRNA than strains that had only the fim or pap operons, respectively ( P = 0·03 and 0·056). Significance and Impact of the Study: Environmental regulation of adhesin expression may be influenced by cross‐talk between fimbrial operons.