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Antimicrobial activity of ultrasound‐assisted solvent‐extracted spices
Author(s) -
Thongson C.,
Davidson P.M.,
Mahakarnchanakul W.,
Weiss J.
Publication year - 2004
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2004.01605.x
Subject(s) - antimicrobial , hexane , chemistry , chromatography , extraction (chemistry) , solvent , salmonella , listeria monocytogenes , food science , antibacterial activity , traditional medicine , essential oil , microbiology and biotechnology , biology , bacteria , biochemistry , organic chemistry , medicine , genetics
Aims: The objective of this research was to determine the antimicrobial activity of conventional and high‐intensity ultrasound‐assisted (HI‐US) solvent‐extracted Thai spices, including ginger ( Zingiber officinale Rose), fingerroot ( Bosenbergia pandurata Holtt) and turmeric ( Curouma longa Linn). Methods and Results: Extracts were obtained using hexane, isopropanol and a 7 : 3 isopropanol : hexane mixture as solvents with and without HI‐US. The antimicrobial activity of the extracts was assayed against four strains each of Listeria monocytogenes and Salmonella Typhimurium DT 104 using an agar dilution assay. Application of HI‐US did not alter antibacterial activity against S. Typhimurium, but antilisterial activity of some HI‐US spice extracts decreased. Solvent type affected antimicrobial efficacy of extracts with hexane producing the least antimicrobial activity. Fingerroot extracted with isopropanol–hexane and without HI‐US had the best antilisterial effect while HI‐US–isopropanol fingerroot extract had the greatest antimicrobial efficacy against S. Typhimurium. Conclusions: Application of HI‐US reduced time of extraction to 5 min, compared with the 24 h required for conventional extraction and maintained antimicrobial activity against Salmonella but slightly reduced activity against Listeria. Significance and Impact of the Study: HI‐US in combination with proper solvent selection may offer a new tool to optimize extraction of spice essential oil for use as antimicrobial agents, and reduce processing time and costs.