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The pattern and kinetics of substrate metabolism of Campylobacter jejuni and Campylobacter coli
Author(s) -
Mohammed K.A.S.,
Miles R.J.,
Halablab M.A.
Publication year - 2004
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2004.01574.x
Subject(s) - campylobacter jejuni , campylobacter , microbiology and biotechnology , biology , bacteria , kinetics , genetics , physics , quantum mechanics
Aims: The main aim was to investigate the patterns and kinetics of substrate oxidation by Campylobacter jejuni and C. coli . Methods and Results: Substrate oxidation profiles by 100 strains were determined using oxygen electrode system. All the isolates tested oxidized formate, l ‐lactate, cysteine, glutamine and serine with high oxidation rates and high affinity but varied in their ability to oxidize citric acid cycle intermediates, aspartic acid and serine. Conclusions: Based on the oxidation ability of α ‐ketoglutarate, succinate, fumarate and aspartic acid, Campylobacter strains tested were divided into three distinct metabolic categories. The first group was able to metabolize α ‐ketoglutarate, succinate, fumarate and aspartic acid; the second group was unable to oxidize α ‐ketoglutarate; and the third group was unable to oxidize, succinate, fumarate, and aspartic acid. Furthermore, serine oxidation rate enabled the differentiation of C. jejuni and C. coli . Significance and Impact of the Study: Overall, the results highlights the extensive metabolic diversity between and within Campylobacter species. In addition, the kinetic data of oxidized substrates obtained may improve the isolation procedures of the organism.