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Comparative analysis of conserved genetic markers and adjacent DNA regions identified in beer‐spoilage lactic acid bacteria
Author(s) -
Suzuki K.,
Ozaki K.,
Yamashita H.
Publication year - 2004
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2004.01572.x
Subject(s) - biology , orfs , lactobacillus brevis , genetics , bacteria , dna , nucleic acid sequence , food spoilage , conserved sequence , dna sequencing , nucleotide , genomic dna , microbiology and biotechnology , lactic acid , peptide sequence , open reading frame , gene , base sequence , lactobacillus plantarum
Aims: To conduct an inter‐species comparative study on the nucleotide sequences of the conserved DNA regions surrounding ORF5, a genetic marker for differentiating beer‐spoilage lactic acid bacteria. Methods and Results: The conserved DNA regions surrounding ORF5 were examined by PCR analysis, using three beer‐spoilage strains, Lactobacillus brevis ABBC45 C , L. paracollinoides LA2 T and Pediococcus damnosus ABBC478. As a result, the DNA regions containing ORF1‐7, originally found in ABBC45 C , appeared to be conserved among the three strains, while the downstream region was not found in L. paracollinoides LA2 T and P. damnosus ABBC478. The sequencing analysis of the conserved DNA regions of LA2 T and ABBC478 revealed ca 99% nucleotide sequence identities with that of ABBC45 C . Conclusions: The nucleotide sequences of the ca 8·2 kb DNA regions containing ORF1‐7 were virtually identical among the three strains belonging to different species. The internal organizations of the ORFs were found to be remarkably similar. Significance and Impact of the Study: The level of nucleotide sequence identities suggests the DNA regions surrounding ORF5 were horizontally acquired by these beer‐spoilage strains belonging to the three different species of lactic acid bacteria.