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Detection of Helicobacter pylori in water by direct PCR
Author(s) -
Benson J.A.,
FodeVaughan K.A.,
Collins M.L.P.
Publication year - 2004
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2004.01555.x
Subject(s) - helicobacter pylori , urease , polymerase chain reaction , contaminated water , digital polymerase chain reaction , biology , microbiology and biotechnology , chemistry , chromatography , urea , environmental chemistry , biochemistry , gene , genetics
Aims: This paper demonstrates a rapid, simple method for the detection of Helicobacter pylori in water that eliminates the need for recovery of cells or DNA extraction prior to PCR. Methods and Results: Direct polymerase chain reaction (DPCR) with primers specific for H. pylori ureA (urease, subunit A) were used to detect H. pylori added to groundwater. DPCR also detected H. pylori in a naturally contaminated water sample. Conclusions: DPCR should provide an improved method to assess contamination of water by H. pylori . Significance and Impact of the Study: This simple, rapid method for detection of H. pylori in water will provide an improved means to investigate the possible role of water as a disease vector.