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Application of electrospray ionization mass spectrometry in rapid typing of fengycin homologues produced by Bacillus subtilis
Author(s) -
Wang J.,
Liu J.,
Wang X.,
Yao J.,
Yu Z.
Publication year - 2004
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2004.01547.x
Subject(s) - bacillus subtilis , electrospray ionization , chemistry , mass spectrometry , chromatography , electrospray , dissociation (chemistry) , collision induced dissociation , bacillaceae , bacillales , tandem mass spectrometry , bacteria , biology , organic chemistry , genetics
Aims: To rapidly type the fengycin homologues produced by Bacillus subtilis strains with electrospray ionization/collision‐induced dissociation (ESI/CID) mass spectrometry. Methods and Results: Fengycin homologues produced by Bacillus subtilis JA were analysed. When each homologue was subjected to ESI/CID analysis, ions representing characteristic fragmentations were detected. These ions can help to identify the homologues; even homologues of the same nominal mass can be discriminated by their ESI/CID spectra. Based on the CID results, fengycin homologues can be correctly assigned. Conclusions and Significance of this Study: ESI/CID leads to rapid detection and structural characterization of fengycin homologues or lipopeptides with similar properties. It will be very useful in studying the regulatory expression of these peptides.