Premium
Bacillus thuringiensis serovar shandongiensis strain 89‐T‐34‐22 produces multiple cytotoxic proteins with similar molecular masses against human cancer cells
Author(s) -
Okumura S.,
Akao T.,
Higuchi K.,
Saitoh H.,
Mizuki E.,
Ohba M.,
Inouye K.
Publication year - 2004
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2004.01544.x
Subject(s) - bacillus thuringiensis , library science , biology , computer science , genetics , bacteria
Aims: To prove that Bacillus thuringiensis serovar shandongiensis strain 89‐T‐34‐22 produces several novel cytotoxic proteins against human leukaemic T cells. Methods and Results: Parasporal inclusion protein was solubilized and processed by proteinase K and was separated by anion‐exchange chromatography. Cytopathic effects of each fraction against MOLT‐4 and Jurkat cells were monitored. Conclusions: Existence of at least two novel cytotoxic proteins was suggested and N‐terminal sequences of the newly identified proteins were determined to be QSTTDVIREY and X (Y or I) (P or I) NLANELA (X indicates uncertain amino acids). Molecular masses of the two proteins were approx. 27–28 kDa. Significance and Impact of the Study: In this study, we demonstrated that the strain 89‐T‐34‐22 produces at least two novel cytotoxic proteins with similar molecular masses against human cancer cells. This is the first strain of B. thuringiensis which produces multiple cytotoxic proteins against human cancer cells.