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The expression of COX2 protein induced by Lactobacillus rhamnosus GG, endotoxin and lipoteichoic acid in T84 epithelial cells
Author(s) -
Korhonen R.,
Kosonen O.,
Korpela R.,
Moilanen E.
Publication year - 2004
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2004.01531.x
Subject(s) - lactobacillus rhamnosus , western blot , microbiology and biotechnology , biology , protein kinase a , lipoteichoic acid , genistein , kinase , p38 mitogen activated protein kinases , biochemistry , lactobacillus , bacteria , endocrinology , gene , fermentation , genetics , staphylococcus aureus
Aims: Lactobacillus rhamnosus GG ( L. rhamnosus GG) possess immunomodulatory effects in the host. In the present study, the effect of L. rhamnosus GG on cyclooxygenase 2 (COX2) expression and its pharmacological control was investigated in human T84 colon epithelial cells. Methods and Results: T84 cells were exposed to freeze‐dried L. rhamnosus GG in vitro , and the expression of COX2 was detected by Western blot. Conclusions: L. rhamnosus GG induced COX2 expression in a time‐ and concentration‐dependent manner in T84 cells. COX2 expression was inhibited by tyrosine kinase inhibitor genistein (100 μ mol l −1 ), p38 mitogen‐activated protein kinase (MAP kinase) inhibitor (SB203580; 1 μ mol l −1 ) and dexamethasone (10 μ mol l −1 ), whereas inhibitors of p42/44 MAP kinase (PD98095; 10 μ mol l −1 ), protein kinase C (Ro 31‐8220; 1 μ mol l −1 ) and transcription factor nuclear factor kappa B (NF‐ κ B) [pyrrolidinedithiocarbamate (PDTC) 100 μ mol l −1 ] had practically no effect. Significance and Impact of the Study: The results provide novel information on the cellular mechanisms involved in the interaction between L. rhamnosus GG and colon epithelial cells.