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Kinetics of enhanced ethanol productivity using raw starch hydrolyzing glucoamylase from Aspergillus niger mutant produced in solid state fermentation
Author(s) -
Rajoka M.I.,
Yasmin A.,
Latif F.
Publication year - 2004
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2004.01526.x
Subject(s) - aspergillus niger , fermentation , chemistry , starch , solid state fermentation , ethanol , mutant , raw material , biochemistry , food science , kinetics , aspergillus , biology , microbiology and biotechnology , organic chemistry , gene , physics , quantum mechanics
Aims: The present investigation deals with the effect of raw starch hydrolyzing glucoamylase by a derepressed mutant of Aspergillus niger on enhanced productivity of ethanol from uncooked starch under non‐aseptic conditions. Methods and Results: The parental culture of Aspergillus niger was improved using γ ‐ray treatment. One derepressed mutant was isolated after extensive screening and optimization and grown on corn cobs, maize starch, soluble starch and wheat bran solid media moistened with Vogel's salts solution and corn steep liquor. The mutant was 2·5‐fold improved over its parent with respect to enzyme productivity, product yield and specific activity. The enzyme from mutated culture was also improved for enzyme properties and could effectively hydrolyze raw starch without the aid of α ‐amylase. Starch hydrolyzed with mutant‐derived glucoamylase supported higher volumetric and product yields of ethanol than those of parental and other strains. Significance and Impact of the Study: The results of the present study are of commercial value. Ethanol product yield coefficient, and volumetric productivity revealed the hyper‐productivity of ethanol from raw starch hydrolyzate obtained with mutant‐derived glucoamylase without addition of liquefying α ‐amylase under non‐aseptic conditions.