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Characterisation of autolytic enzymes in Lactobacillus pentosus
Author(s) -
Cibik R.,
ChapotChartier M.P.
Publication year - 2004
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2004.01516.x
Subject(s) - autolysis (biology) , peptidoglycan , lactobacillus , microbiology and biotechnology , lactobacillaceae , biology , bacteria , enzyme , food science , chemistry , biochemistry , genetics
Aims: To characterize autolysis and autolytic system of the lactic acid bacterium Lactobacillus pentosus. Methods and Results: Autolysis of nine Lact. pentosus strains was evaluated in buffer solution. Their peptidoglycan hydrolase profiles were examined by renaturing SDS‐PAGE and revealed two major activity bands at 58 and 112 kDa. Specificity analysis indicated the presence of at least two different types of peptidoglycan hydrolase activities in Lact. pentosus 1091. Conclusions: Autolysis of Lact. pentosus was shown to be strain dependent and involvement of at least two different autolysins was evidenced. Significance and Impact of the Study: The autolytic system of Lact. pentosus was characterized for the first time and the data obtained could be used in the selection of strains of technological interest.