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In vivo characterization of Lactobacillus johnsonii FI9785 for use as a defined competitive exclusion agent against bacterial pathogens in poultry
Author(s) -
La Ragione R.M.,
Narbad A.,
Gasson M.J.,
Woodward M.J.
Publication year - 2004
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2004.01474.x
Subject(s) - biology , microbiology and biotechnology , clostridium perfringens , salmonella enteritidis , competitive exclusion , colonization , pathogen , salmonella enterica , lactobacillus , escherichia coli , salmonella , bacteria , competition (biology) , ecology , biochemistry , genetics , gene
Aims: To test the efficacy of Lactobacillus johnsonii FI9785 in reducing the colonization and shedding of Salmonella enterica serotype Enteritidis, Escherichia coli O78:K80 and Clostridium perfringens in poultry. Methods and Results: Specific pathogen‐free chicks (1 day old) were dosed with a single oral inoculum of 1 × 10 9 CFU. Lactobacillus johnsonii FI9785 and 24 h later were challenged in separate experiments with S . Enteritidis (S1400, nal r ) and E. coli O78:K80 (EC34195, nal r ). There were no significant effects against S . Enteritidis whereas colonization of the small intestine by E. coli O78:K80 was reduced significantly. Both S . Enteritidis and E. coli colonized the caeca and colon to levels equivalent to control birds and there was no reduction in shedding as assessed by a semi‐quantitative cloacal swabbing technique. Specific pathogen‐free chicks (20 day old) were dosed with a single oral inoculum of 1 × 10 9 CFU L. johnsonii FI9785 and 24 h later were challenged with C. perfringens . A single oral dose of L. johnsonii FI9785 was sufficient to suppress all aspects of colonization and persistence of C. perfringens . Conclusions: Lactobacillus johnsonii FI9785 may be given to poultry for use as a competitive exclusion agent to control C. perfringens . Significance and Impact of the Study: Lactobacillus johnsonii FI9785 may be a valuable tool to control the endemic disease of necrotic enteritis, thereby reducing economic losses associated with reduced use of antimicrobials in the poultry industry.