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Rapid identification and differentiation of Saccharomyces cerevisiae , Saccharomyces bayanus and their hybrids by multiplex PCR
Author(s) -
Torriani S.,
Zapparoli G.,
Malacrinò P.,
Suzzi G.,
Dellaglio F.
Publication year - 2004
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2004.01468.x
Subject(s) - biology , multiplex polymerase chain reaction , saccharomyces cerevisiae , saccharomyces , multiplex , microbiology and biotechnology , genetics , polymerase chain reaction , yeast , gene
Aims: To develop a multiplex PCR assay for the specific identification and differentiation of Saccharomyces cerevisiae , S. bayanus and their hybrids. Methods and Results: Two sets of primers with sequences complementary to the region YBR033w were used. A single amplicon of 1710 bp or 329 bp was obtained with species S. cerevisiae and S. bayanus , respectively, while the presence of both bands was observed in S. pastorianus because of its hybrid nature. Both amplification products were also obtained after amplification from DNA of several laboratory S. cerevisiae × S. bayanus hybrid strains. Conclusions: Multiplex PCR was optimized for the rapid and reliable identification of S. cerevisiae, S. bayanus and their hybrids. Significance and Impact of the Study: The procedure may be used for routine detection of the most common Saccharomyces sensu stricto yeasts involved in industrial fermentation processes, overcoming the problems of conventional techniques.