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Novel method for cell immobilization and its application for production of oligosaccharides from sucrose
Author(s) -
Shin H.T.,
Park K.M.,
Kang K.H.,
Oh D.J.,
Lee S.W.,
Baig S.Y.,
Lee J.H.
Publication year - 2004
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2003.01480.x
Subject(s) - sucrose , production (economics) , chemistry , oligosaccharide , biochemistry , microbiology and biotechnology , biology , economics , macroeconomics
Aims: The purpose of the present investigation was to develop a novel method for cell immobilization. Methods and Results: Aureobasidium pullulans cells were mixed with an alginate solution, and the mixture was extruded to form small gel beads as hydrated‐immobilized cells. The beads were then placed at −15°C for 6–24 h to induce freeze‐dehydration. The freeze‐dehydration resulted in shrinkage of beads as a result of water removal reducing bead volume by 82% and bead weight by 85%. The dehydrated beads were successfully used for the production of fructo‐oligosaccharides in a model reactor system. Conclusions: Dehydrated beads may provide some commercial advantages over conventional immobilized cells. Significance and Impact of the Study: This study shows that bioreactor performance can be improved up to two times by the use of the dehydrated beads.