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A simple and easy method for the monitoring of environmental pollutants using oligotrophic bacteria
Author(s) -
Tada Y.,
Kobata T.,
Nakaoka C.
Publication year - 2001
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2001.00850.x
Subject(s) - sphingomonas paucimobilis , environmental chemistry , pollutant , heavy metals , environmental science , bacteria , contamination , sphingomonas , metal , chemistry , ecology , biology , genetics , pseudomonas , organic chemistry
Y. TADA, T. KOBATA AND C. NAKAOKA. 2001 . Sphingomonas paucimobilis KPS01, an oligotrophic bacterium isolated from soil, may be a useful tool for monitoring heavy metals. Previous methods relying on counting of viable cells require a relatively long time and some skill; we have developed a method based on optical density (O.D.) measurements which is significantly faster and does not require skilled personnel. The results of the O.D. and viable count methods were consistent; both methods detected heavy metals at concentrations ranging from 10 −3 to 10 −5 mmol l −1 and identified heavy metal contamination in 13 of 18 river water samples. Our results demonstrate that biological detection using this O.D. method and S. paucimobilis KPS01 may be useful for routine environmental monitoring of heavy metals, particularly in water sources.