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Detection of Escherichia coli O157 in French food samples using an immunomagnetic separation method and the VIDAS TM E. coli O157
Author(s) -
VernozyRozand C.,
Mazuy C.,
RayGueniot S.,
BoutrandLoeï S.,
Meyrand A.,
Richard Y.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1997.tb00014.x
Subject(s) - immunomagnetic separation , escherichia coli , cefixime , raw milk , food science , immunoassay , sorbitol , contamination , enterobacteriaceae , biology , agar , salmonella , chromatography , food contaminant , microbiology and biotechnology , chemistry , bacteria , biochemistry , antibody , ecology , genetics , cephalosporin , immunology , gene , antibiotics
C. VERNOZY‐ROZAND, C. MAZUY, S. RAY‐GUENIOT, S. BOUTRAND‐LOEï, A. MEYRAND AND y. richard. 1997. Two commercially available screening methods, an automated enzyme‐linked fluorescent immunoassay (VIDAS TM E. coli O157) and an immunomagnetic separation followed by culture onto cefixime tellurite sorbitol MacConkey agar (CT‐SMAC), were compared for detection of Escherichia coli O157 in naturally and artificially contaminated food samples. A total of 250 naturally contaminated food samples, including raw milk cheeses, poultry, raw sausages and ground beef retail samples, were examined. Four poultry, one raw sausage and one ground beef sample were found to be positive for E. coli O157 by both methods. Of the six positive samples, five were shown to contain sorbitol‐positive, O157‐positive, H7‐negative, motile and non‐verotoxin‐producing E. coli .