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Characterization of urethanase from Micrococcus species associated with the marine sponge ( Spirasfrella species)
Author(s) -
Mohapatra B.R.,
Bapuji M.
Publication year - 1997
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1997.tb00003.x
Subject(s) - sponge , enzyme , arrhenius plot , micrococcus , chemistry , enzyme assay , ethanol , bacteria , environmental chemistry , biology , stereochemistry , microbiology and biotechnology , food science , botany , biochemistry , organic chemistry , activation energy , genetics
B.R. Mohapatra and M. Bapuji. 1997. Eighty per cent of the urethanase activity of Micrococcus sp. isolated from the marine sponge Spirastrella sp. was cell associated. The urethanase had optimal activity at pH 5.0 and 45 °C. The activation and deactivation energies of the partially purified enzyme, calculated from an Arrhenius plot, were 9.31 and 34.01 kcal mol −1 , respectively. The enzyme was not affected by EDTA and the major cations of sea water, such as Ca 2+ , Mg 2+ and Na + . The enzyme was resistant to 20% (v/v) ethanol and may be practically applicable in the removal of urethane from alcoholic beverages.