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The use of a Rapid Salmonella Latex Serogrouping Test (Spectate) to assist in the confirmation of ELISA‐based Rapid Salmonella Screening tests
Author(s) -
Cheesbrough S.,
Donnelly C.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb01183.x
Subject(s) - salmonella , microbiology and biotechnology , enterobacteriaceae , virology , biology , bacteria , escherichia coli , biochemistry , genetics , gene
S. CHEESBROUGH AND C. DONNELLY. 1996. Spectate, a 10 min, simple, latex‐based agglutination test for serogrouping of salmonellae, has been investigated as a tool to assist in the confirmation of ELISA presumptive positive broth samples when screening for salmonellae in foods. When obtaining a combined ELISA and Spectate positive result, there was a 90% (27/30) confidence limit of a genuine positive result, some one or two working days quicker than the traditional methods. Of the 10% (3/30) that were not confirmed as salmonellas, two gave a Spectate result which is advised as being a possible Citrobacter spp. and one sample gave a positive confirmation by Spectate only, which suggested a failure of the traditional confirmation process, a finding confirmed at other sites. Extensive studies performed at several food company microbiology laboratories showed Spectate to be a useful additional tool in the confirmation process of ELISA screening techniques for salmonellae in food. Additionally the concept of a ‘false positive’ may need to be refined to that of a ‘not culturally’ positive, given the apparent possible failure of the traditional confirmation methods.