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Detection of Bacteroides fragilis by PCR assay targeting the neuraminidase‐encoding gene
Author(s) -
Kuwahara T.,
Akimoto S.,
Ugai H.,
Kamogashira T.,
Kinouchi T.,
Ohnishi Y.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb01179.x
Subject(s) - bacteroides fragilis , biology , neuraminidase , oligomer restriction , gene , microbiology and biotechnology , nested polymerase chain reaction , bacteroidaceae , polymerase chain reaction , oligonucleotide , virology , bacteria , genetics , virus , antibiotics
T. KUWAHARA, S. AKIMOTO, H. UGAI, T. KAMOGASHIRA, T. KINOUCHI AND Y. OHNISHI. 1996. Oligonucleotide primers were designed on the basis of the sequence of the neuraminidase‐encoding gene ( nanH ) of Bacteroides fragilis and used for the specific detection of this anaerobe by the nested PCR assay. Fifty‐nine of 60 representative strains of Bact. fragilis were detected, while none of 45 strains of other species generated visible PCR products. The detection limits of Bact. fragilis cells and DNA by the nested PCR were 10 colony‐forming units and 10 fg of chromosomal DNA, respectively. The PCR assay targeting the nanH gene has the potential for the detection of Bact. fragilis .