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Glycerol dehydratase activity: the limiting step for 1,3‐propanediol production by Clostridium butyricum DSM 5431
Author(s) -
AbbadAndaloussi S.,
Guedon E.,
Spiesser E.,
Petitdemange H.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb01168.x
Subject(s) - clostridium butyricum , chemostat , biochemistry , glycerol , 1,3 propanediol , catabolism , butyrate , chemistry , propanediol , dehydratase , dehydrogenase , biology , fermentation , enzyme , bacteria , organic chemistry , genetics
S. ABBAD‐ANDALOUSSI, E. GUEDON, E. SPIESSER AND H. PETITDEMANGE. 1996. Glycerol catabolism by Clostridium butyricum DSM 5431 into acetate, butyrate and 1,3‐propanediol (1,3‐PD) was studied in chemostat culture. The fact that the intracellular concentrations of NADH (18–22 μUmol g ‐1 dry cell mass) were extremely high suggested that the dehydratase activity was the rate limiting step in 1,3‐PD formation. This limitation was proved by the addition of propionaldehyde, another substrate of propanediol dehydrogenase, into the culture medium. This resulted in an increase in (i) glycerol utilization, (ii) biomass formation and (iii) product biosynthesis.