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Three‐dimensional visualization of Salmonella attachment to poultry skin using confocal scanning laser microscopy
Author(s) -
Kim K.Y.,
Frank J.F.,
Craven S.E.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb01161.x
Subject(s) - confocal laser scanning microscopy , confocal , laser scanning , microscopy , confocal microscopy , laser microscopy , visualization , laser , salmonella , biology , poultry meat , materials science , optics , microbiology and biotechnology , bacteria , computer science , artificial intelligence , food science , physics , genetics
K.Y. KIM, J.F. FRANK AND S.E. CRAVEN. 1996. The objective of this study was to locate the position of attached or entrapped Salmonella cells in poultry skin. Confocal scanning laser microscopy (CSLM) was used to obtain optical sections of intact poultry skin without artefacts associated with dehydration and other sample preparation techniques. A technique was developed to prevent compression of the poultry skin during CSLM operation. Images of bacteria and poultry skin were obtained after staining with Pyronin‐Y. Data indicated that Salmonella cells were mostly located in the cervices and feather follicles. Salmonella in feather follicle floated freely in surrounding liquid even after the skin was thoroughly rinsed.