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2‐Chlorobenzoic acid and 2,5‐dichlorobenzoic acid metabolism by crude extracts of Pseudomonas sp. CPE2 strain
Author(s) -
Fava F.,
Baldoni F.,
Marchetti L.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb01160.x
Subject(s) - catechol , strain (injury) , oxygen , metabolism , pseudomonas , chemistry , pseudomonadaceae , pseudomonadales , biochemistry , nuclear chemistry , stereochemistry , bacteria , biology , organic chemistry , genetics , anatomy
F. FAVA, F. BALDONI AND L. MARCHETTI. 1996. Crude extracts of Pseudomonas sp. CPE2 strain, which is capable of growing on 2‐chlorobenzoic acid (2–CBA) and 2,5–dichlorobenzoic acid (2,5–dCBA) in the absence of other carbon sources, were found to be capable of bioconverting 2–CBA and 2,5–dCBA to catechol and 4–chlorocatechol, respectively, by a reaction requiring molecular oxygen and exogenous NADH. Extracts obtained from 2–CBA‐grown cells in the presence of 2–CBA and from 2,5–dCBA‐grown cells in the presence of 2,5–dCBA were found to have activities similarly influenced by the assay parameters pH, temperature, and by concentration of oxygen, protein, Fe 2+ , FAD and NADH in the assay medium. In addition, the activity of the two crude extracts in the presence of 2–CBA or 2,5–dCBA was described by very similar Michaelis‐Menten kinetic parameters. These observations led to the speculation that a unique broad‐spectrum chlorobenzoate 1,2–dioxygenase catalyses the 2–CBA and 2,5–dCBA metabolism both in 2–CBA‐and 2,5–dCBA‐grown CPE2 cells.