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Instability of plasmid‐encoded citrate permease in Leuconostoc
Author(s) -
Kihal M.,
Prévost H.,
Lhotte M.E.,
Huang D.Q.,
Diviès C.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb01147.x
Subject(s) - leuconostoc mesenteroides , plasmid , permease , mutant , biology , phenotype , microbiology and biotechnology , leuconostoc , gene , dna , biochemistry , chemistry , genetics , bacteria , lactic acid
M. KIHAL, H. PRÉVOST, M.E. LHOTTE, D.Q. HUANG AND C. DIVIÈS. 1996. The conversion from citrate positive (Cit + ) to citrate negative (Cit ‐ ) phenotype of six strains of Leuconostoc mesetiteroides was followed during growth in milk and buffered or unbuffered MRS medium at 30 or 37°C. High rate of loss of Cit + phenotype was observed. The Cit ‐ phenotype was found to be linked to the loss of 22 to 23 kb plasmids. All Cit ‐ mutants isolated from Leuc. mesenteroides subsp. cremoris 195 reverted spontaneously to the Cit + phenotype. Hybridization experiments using a 0.8 kb fragment of the citP gene of Leuc. mesenteroides showed that all the plasmids which were lost in Cit ‐ mutants encoded for a citrate permease. However, neither plasmid nor genomic DNA from Leuc. mesenteroides subsp. cremoris 195 hybridized with the citP probe.