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Transformation of Streptococcus bovis protoplasts by plasmid DNA
Author(s) -
Mareková M.,
Kmet V.,
Javorský P.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb01132.x
Subject(s) - protoplast , transformation (genetics) , plasmid , streptococcus bovis , microbiology and biotechnology , sucrose , agar , biology , strain (injury) , dna , polyethylene glycol , bacteria , peg ratio , agar plate , transformation efficiency , biochemistry , genetics , fermentation , agrobacterium , gene , rumen , finance , anatomy , economics
M. MAREKOVÁ, V. KMET' AND P. JAVORSKÝ. 1996. The transformation and subsequent regeneration of ruminal strain Streptococcus bovis AO24/85 protoplasts by plasmid DNA was studied. The best stabilizer for regeneration of protoplasted cells was 5% sucrose in the regeneration medium and in the agar plates. Optimal concentration of polyethylene glycol 6000 in the transformation medium was 25% for both plasmids tested. Addition of Ca 2+ and Mg 2+ ions (2.5 mmol l ‐1 ) to the transformation medium increased the proportion of regenerated cells. Transformation frequencies were 3 times 10 3 transformants per μg of pNZ12 and 2.4 times 10 2 per μg of pJK108, respectively.