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A partially purified β‐glucosidase from Bifidobacterium adolescentis converts cycasin to a mutagenic compound
Author(s) -
Choi Y.J.,
Kim C.J.,
Ji G.E.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb01129.x
Subject(s) - mutagen , ames test , enzyme , chemistry , bifidobacterium , biochemistry , size exclusion chromatography , actinomycetaceae , chromatography , ion chromatography , bacteria , biology , carcinogen , salmonella , lactobacillus , fermentation , genetics
Y.J. CHOI, C.J. KIM AND G.E. JI. 1996. β‐Glucosidase was extracted from sonicated Bifidobacterium adolescentis Int‐57 and partially purified by Sepharose CL‐6B gel‐filtration and DEAE‐cellulose ion‐exchange chromatography. The partially purified enzyme was confirmed to convert cycasin to a mutagen in the Ames and SOS chromotests. β‐Glucosidase negative strains were unable to activate cycasin mutagenically.