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A new monoclonal antibody detecting ochratoxin A at the picogram level
Author(s) -
GyöngyösiHorváth Á.,
BarnaVetró I.,
Solti L.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb01119.x
Subject(s) - ochratoxin a , ochratoxin , monoclonal antibody , antibody , chemistry , immunization , immunoglobulin light chain , subclass , monoclonal , microbiology and biotechnology , ochratoxins , chromatography , mycotoxin , biology , immunology , food science
Á. GYÖNGYÖSI‐HORVÁTH, I. BARNA‐VETRÓ AND L. SOLTI. 1996. A monoclonal antibody against ochratoxin A was produced after immunization of Balb/c mice with ochratoxin A‐BSA. This antibody was of the IgGl heavy chain subclass with a k type light chain. The 50% inhibition value was 0.45 ng ml ‐1 in a direct competitive ELISA and the detection limit was 42 pg ml ‐1 . This antibody is very specific, cross‐reacting only with ochratoxin B (9.3%).