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Cloning of a cellulase gene from the rumen anaerobe Fibrobacter succinogenes SD35 and partial characterization of the gene product
Author(s) -
Ozcan N.,
Cunningham C.,
Harris W.J.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb01114.x
Subject(s) - fibrobacter succinogenes , cellulase , biochemistry , nucleic acid sequence , molecular cloning , molecular mass , gene , cloning (programming) , biology , gene product , microbiology and biotechnology , peptide sequence , enzyme , rumen , chemistry , gene expression , fermentation , computer science , programming language
N. OZCAN, C. CUNNINGHAM AND W.J. HARRIS. 1996. A gene encoding an enzyme which degrades cellulose ( end‐1 ) was isolated from a library of Fibrobacter succinogenes SD35 DNA fragments and expressed in pUC18. The product of end‐1 showed significant activity against carboxymethylcellulose but relatively minor activity against lichenan, xylan and avicel. The nucleotide sequence indicated a product of 388 amino acids with a molecular mass of 50.2 kDa. This was in agreement with the molecular size estimated by gel electrophoresis. No significant DNA sequence similarity was identified with any published endoglucanase.