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Effect of penicillin G on the electroporation of Rhodococcus rhodochrous CF222
Author(s) -
Sunairi M.,
Iwabuchi N.,
Murakami K.,
Watanabe F.,
Ogawa Y.,
Murooka H.,
Nakajima M.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb01110.x
Subject(s) - rhodococcus rhodochrous , penicillin , microbiology and biotechnology , electroporation , rhodococcus , biology , bacteria , chemistry , antibiotics , biochemistry , genetics , gene
M. SUNAIRI, N. IWABUCHI, K. MURAKAMI, F. WATANABE, Y. OGAWA, H. MUROOKA AND M. NAKAJIMA. 1996. Suitable conditions for the introduction of bacteriophage DNA into cells of Rhodococcus rhodochrous CF222 by electroporation were established, and penicillin G was found to enhance the transfection frequency. When conditions optimal for the parental strain were applied to its colony‐morphological mutants, different transfection frequencies were observed. Penicillin G enhanced the transfection frequency of smooth and mucoidal mutants but not of rough mutants.