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Degradation of phenol through ortho ‐cleavage pathway by Pseudomonas stutzeri strain SPC2
Author(s) -
Ahamad P.Y. Aneez,
Kunhi A.A.M.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb01101.x
Subject(s) - pseudomonas stutzeri , catechol , phenol , strain (injury) , pseudomonas , chemistry , metabolic intermediate , pseudomonadaceae , degradation (telecommunications) , pseudomonadales , biodegradation , metabolic pathway , bacteria , cleavage (geology) , dioxygenase , stereochemistry , enzyme , biochemistry , microbiology and biotechnology , organic chemistry , biology , telecommunications , paleontology , genetics , anatomy , fracture (geology) , computer science
P.Y. ANEEZ AHAMAD AND A.A.M. KUNHI. 1996. Generally pseudomonads degrade phenol through the meta ‐pathway, but Pseudomonas stutzeri strain SPC2 isolated by flask enrichment of municipal sewage degraded phenol through the ortho ‐pathway. The strain utilized up to 1200 ppm of phenol as a sole source of carbon and energy. The strain also degraded benzoate and 4‐hydroxy and 3,4‐dihydroxybenzoates via the ortho ‐pathway. Cell‐free extracts of the strain grown on these substrates showed fairly good catechol 1,2‐dioxygenase (C1,2‐D) and protocatechuate 3,4‐dioxyenase (PCA 3,4‐D) activities, the induction of both activities being increased by benzoate. No meta ‐cleavage activities were detected.