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Detection of Campylobacter jejuni and Camp. coli in chicken faecal samples by PCR
Author(s) -
Rasmussen H.N.,
Olsen J.E.,
Jørgensen K.,
Rasmussen O.F.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb00209.x
Subject(s) - campylobacter jejuni , flagellin , biology , microbiology and biotechnology , polymerase chain reaction , campylobacter coli , campylobacter , escherichia coli , bacteria , gene , genetics
H.N. RASMUSSEN, J.E. OLSEN, K. JØRGENSEN AND O.F. RASMUSSEN. 1996. PCR primers were selected from the flagellin gene sequences flaA and flaB of Campylobacter coli to amplify DNA from Camp. jejuni and Camp. coli. When the PCR products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of Camp. jejuni and Camp. coli. The assay was used to analyse 31 chicken faecal samples. Full correspondence was found between the PCR assay conducted on the enriched cultures and the standard culture method. When analysing the transport medium prior to enrichment, the PCR assay detected nine of 11 culture positive samples.