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Detection of Hsp60 (GroEL)‐like proteins in Vibrio parahaemolyticus and vibrio species by Western immunoblotting analysis
Author(s) -
Koga T.,
Nakajyo Y.,
Komoto A.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb00193.x
Subject(s) - vibrio parahaemolyticus , microbiology and biotechnology , clinical microbiology , groel , biology , library science , bacteria , escherichia coli , gene , genetics , computer science
Detection of heat shock proteins in Vibrio parahaemolyticus was investigated by SDS‐PAGE and Western immunoblotting procedure using an anti‐Hsp 60 antibody. Results indicate that V. parahaemolyticus elicited at least one Hsp 60 (GroEL)‐like protein with apparent molecular weight of about 58 000 (58 kDa) when submitted to a heat shift from 30 to 42d̀C. Kanagawa phenomenon‐positive and ‐negative strains of V. parahaemolyticus responded the same way. Six other Vibrio species also showed an increased synthesis of GroEL‐like (58 kDa) protein after heat shock, while synthesis of 58 kDa protein of V. alginolyticus was at a similar level before and after heat shock. Vibrio nereis showed an increased synthesis of a 60 kDa GroEL‐like protein.