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Studies of Clostridium cellulolyticum ATCC 35319 under dialysis and co‐culture conditions
Author(s) -
Gehin A.,
Cailliez C.,
Petitdemange E.,
Benoit L.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb00067.x
Subject(s) - library science , humanities , art , computer science
A. Gehin, C. Cailliez, E. Petitdemange And L. Benoit. 1996. The degradation of cellulose by Clostridium celulolyticum has been studied in several ways; (1) in batch fermentation in 50‐ml sealed‐cap flasks, referred to as the control; (2) in batch fermentation with pH at 7.2; (3) fermentation in dialysis which permits elimination of all the products of metabolism; (4) fermentation in dialysis with a constant bubbling of nitrogen; (5) in co‐culture with Clostridium A22 in batch with and without pH regulation and with dialysis. H 2 , CO 2 , acetate, ethanol and lactate were the major end‐products of cellobiose and cellulose fermentation. Compared to batch culture, growth of CI. cellulolyticum on cellobiose increased by a factor of 10 in dialysed culture. The end products from the dialysed culture were detected in a small range compared to the concentration for the batch culture. Related to the biomass, CMCase activities were of the same level, showing a direct relation between the biomass formation and the cellulase production. The percentage of cellulose degradation (50%) by CI. cellulolyticum was greater when dialysis of end products with a constant bubbling of nitrogen took place during the course of fermentation (6 d) in comparison with cultures in 50‐ml sealedcap flasks (23%), in a fermentor (36%) or using dialysis without N 2 bubbling (40%). The presence of two micro‐organisms produced no further enzyme activities and hence the percentage of cellulose degradation was quite similar in mono‐ and co‐culture. No synergistic action was found between two cellulolytic strains.