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Sub‐typing of animal and human Campylobacter spp. using RAPD
Author(s) -
Madden R.H.,
Moran L.,
Scates P.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb00056.x
Subject(s) - rapd , typing , biology , campylobacter , microbiology and biotechnology , genetics , bacteria , genetic diversity , population , medicine , environmental health
R.H. MADDEN, L. MORAN AND P. SCATES. 1996. Based on a 10‐mer primer (5′‐ CCTGTTAGCC‐3′), a random amplified polymorphic DNA (RAPD) method for typing Campylobacter coli isolated from pigs was developed. The method proved effective with a high discrimination and good reproducibility. In contrast with serotyping no untypable strains were found out of a total of 269 isolates (veterinary, food and clinical) examined. The method was also successfully applied to typing Campylobacter jejuni from a similar range of sources.