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Protoplasts from pectinolytic fungi: isolation, regeneration and pectinolytic enzyme production
Author(s) -
Solís S.,
Flores M.E.,
Huitrón C.
Publication year - 1996
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1996.tb00024.x
Subject(s) - protoplast , isolation (microbiology) , biology , enzyme , pectinase , fungi imperfecti , microbiology and biotechnology , regeneration (biology) , botany , bacteria , biochemistry , genetics
S. Solís, M.E. FLORES AND C. HUITRON. 1996. Protoplast release in pectinolytic strain mutants of Aspergillus sp. CH‐Y‐1043 (A13) and Aspergillus flavipes ATCC‐16795 (F7) is described. Optimum yield of protoplasts A13 was obtained in a lapse of 1 h when commercially lytic enzymes of Trichoderma harzanium (2 mg ml −1 ) were added in 0.05 mol 1 −1 citrate‐phosphate buffer pH 5.0 containing 0.7 mol 1 −1 KCl and 10 mg ml −1 BSA. Best results in F7 were obtained when the protoplasting system of A13 was supplemented with 10 mg ml −1 Aureobasidium sp. lytic enzymes. Isolated protoplasts in A13 and F7 were capable of a high regeneration frequency of 87% and 53% when 0.7 mol 1 −1 KCl and sorbitol were used as osmotic stabilizers. Endo‐P, Exo‐P and pectin lyase production were not modified during the process of regeneration.