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X‐ray microanalysis of chlorhexidine‐treated cells of Saccharomyces cerevisiae
Author(s) -
Hiom S.J.,
Hann A.C.,
Furr J.R.,
Russell A.D.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01319.x
Subject(s) - vacuole , saccharomyces cerevisiae , cytoplasm , yeast , chlorine , chlorhexidine , microanalysis , microbiology and biotechnology , saccharomyces , chemistry , cell , quantitative analysis (chemistry) , biology , biochemistry , chromatography , medicine , dentistry , organic chemistry
The use of energy dispersive analysis of X‐rays (EDAX) to identify and quantify the presence of chlorhexidine diacetate (CHA) within Saccharomyces cerevisiae cells was examined. Chlorine was used as the elemental marker tag. Saccharomyces cerevisiae cells exposed to 1000 μg ml ‐1 CHA took up increasing amounts of CHA over a time period of 30 s to 30 min. Electron probe micro‐analysis was employed to examine the specific accumulation of CHA across the treated cells. These results showed that CHA was distributed evenly between the cell wall, cytoplasm and vacuoles of cells pre‐treated with this concentration of CHA for 30 min. The EDAX system therefore provides a useful tool for examining the qualitative and quantitative effects of chlorhexidine on yeast cells, although quantitative data must be interpreted with caution.