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Anaerobic degradation of pectin by mixed consortia and optimization of fermentation parameters for higher pectinase activity
Author(s) -
Shivakumar P.D.,
Nand Krishna
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01300.x
Subject(s) - pectinase , pectin , pectinesterase , cellulase , fermentation , food science , anaerobic exercise , xylanase , chemistry , pectin lyase , biology , cellulose , biochemistry , enzyme , physiology
Samples from biogas digesters, sewage ponds, animal house effluents and food processing wastes were used in enrichment systems seeking anaerobic bacteria producing pectinases. Among the 46 anaerobic consortia developed from various samples, four showed high pectinase activity under static anaerobic conditions. Investigation of fermentation variables showed the optimum conditions for pectinase activity were pH 7.0, 45°C and 72 h of growth with 0.5% pectin in the cultivation medium. A 1.4‐ to 1.6‐fold increase in the pectinase activity was achieved under these conditions. The maximum yield of enzymes (62.72 U ml‐1 of pectinase, 4.74 U ml ‐1 of polygalacturonase, 113.30 U ml ‐1 of pectin lyase, 2.10 U ml ‐1 of pectinesterase, 0.75 U ml ‐1 of total cellulase and 9.27 U ml ‐1 of xylanase) was recorded with the consortia C‐S2 developed from decomposed plant samples collected from a pond.