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Biochemical studies on the cell wall degradation of Fusarium oxysporum f. sp. lycopersici race 2 by its own lytic enzymes for its biocontrol
Author(s) -
Alfonso C.,
Santamaría F.,
Nuero O.M.,
Prieto A.,
Leal J.A.,
Reyes F.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01297.x
Subject(s) - cell wall , chitin , lysis , fusarium oxysporum , polysaccharide , mannan , fusarium oxysporum f.sp. lycopersici , enzymatic hydrolysis , biochemistry , chemistry , hydrolysis , lytic cycle , cellulase , autolysis (biology) , chromatography , enzyme , biology , botany , fusarium wilt , virus , virology , chitosan
An exhaustive cell wall fractionation of Fusarium oxysporum f. sp. lycopersici race 2 ( Fol 2) with alkali in a sequential procedure yields only three polysaccharide fractions: F1 s (alkali and water soluble), F1 1 (alkali soluble and water insoluble) and F4 (alkali‐insoluble residue). These fractions amounted respectively to 15, 1.3 and 52% of the cell wall and have been characterized by infra‐red spectroscopy and gas liquid chromatography‐mass spectrometry (GLC‐MS). F1 s is a β‐gluco‐galacto‐mannan, F1 1 is mainly composed of a β‐1, 3‐glucan and F4 is a β‐1,3‐glucan‐chitin complex. The F1 s is a very complex polysaccharide and its hydrolysis requires the action of different enzymes. The lysis of the cell wall and its three fractions with lytic enzymes from Fol 2 has been studied and a correlation between the lysis of the cell wall and the lysis of these fractions was found. The amount of glucose, galactose and mannose in F1 s and cell wall hydrolysates were quantified by GLC and they indicated the hydrolysis of the gluco‐galacto‐mannan polysaccharide. In the hydrolysis of F4 and cell walls N ‐acetylglucosamine was also found and quantified. When chlamydospores of this fungus were treated with Fol 2 lytic enzymes, the sugars liberated were principally mannose and N ‐acetylglucosamine. These results indicate that Fol 2 produces during its autolysis the necessary enzymes to hydrolyse its own cell walls. This fact suggests that a biological control of Fol 2 with its own lytic enzymes, conveniently immobilized, could be developed.