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Characterization of L‐asparaginase from Bacillus sp. isolated from an intertidal marine alga ( Sargassum sp.)
Author(s) -
Mohapatra B.R.,
Sani R. K.,
Banerjee U.C.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01086.x
Subject(s) - intertidal zone , sargassum , algae , bacillales , enzyme , biology , bacteria , enzyme assay , extracellular , microbiology and biotechnology , bacillaceae , bacillus (shape) , botany , biochemistry , ecology , bacillus subtilis , genetics
B.R. MOHAPATRA, R.K. SANI AND U.C. BANERJEE. 1995. The bacterial flora associated with an intertidal marine alga ( Sargassum sp.) were screened for the presence of extracellular L‐asparaginase; one out of five Bacillus strains was found positive. The maximum L‐asparaginase activity was found at 37°C and pH 8.0. The optimum NaCl concentration for enzyme activity was found to be 2% (w/v). The enzyme activity was not affected by the addition of different metal ions (Ca 2+ , Co 2+ , Fe 2+ , Mg 2+ and Ni 2+ ) at 10 mmol 1 ‐1 , but was strongly inhibited by EDTA.