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Development of RAPD protocol for typing of strains of lactic acid bacteria and enterococci
Author(s) -
Cocconcelli P.S.,
Porro D.,
Galandini S.,
Senini L.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01085.x
Subject(s) - rapd , enterococcus faecium , typing , biology , streptococcus thermophilus , microbiology and biotechnology , bacteria , enterococcus , enterococcus faecalis , lactobacillus reuteri , primer (cosmetics) , lactobacillus helveticus , lactic acid , lactobacillus acidophilus , streptococcaceae , lactobacillus , genetics , probiotic , chemistry , medicine , population , environmental health , organic chemistry , genetic diversity , staphylococcus aureus
P.S. COCCONCELLI, D. PORRO, S. GALANDINI AND L. SENINI. 1995. A protocol for typing strains of lactic acid bacteria and enterococci based on randomly amplified polymorphic DNA (RAPD) fragments has been developed. Using a single 10‐mer primer, fingerprints were achieved without the need to isolate genomic DNA. Different conditions of DNA release and amplification were investigated in order to obtain reproducible results and high discrimination among strains. This RAPD protocol was successfully applied for the typing of strains belonging to the species Lactobacillus acidophilus, Lact. helveticus, Lact. casei, Lact. reuteri, Lact. plantarum, Enterococcus faecalis, Ent. faecium and Streptococcus thermophilus.