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Isolation of uracil auxotrophs from Burkholderia cepacia ATCC 25416
Author(s) -
Li K.,
West T.P.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01074.x
Subject(s) - auxotrophy , uracil , biology , burkholderia , biochemistry , microbiology and biotechnology , mutant , aspartate carbamoyltransferase , enzyme , bacteria , dna , genetics , allosteric regulation , gene
K. LI AND T.P. WEST. 1995. Two uracil auxotrophs of the phytopathogen Burkholderia cepacia ATCC 25416, which is known to be involved in food spoilage, were isolated by a combination of ethylmethane sulphonate and D‐cycloserine counterselection. One mutant exhibited depressed orotate phosphoribosyltransferase activity while the other mutant lacked orotidine 5'‐monophosphate decarboxylase activity. Pyrimidine limitation of either auxotroph elevated aspartate transcarbamoylase and dihydroorotase activities by at least 1.5‐fold indicating that these pathway enzymes may be repressible by a uracil‐related compound in B. cepacia . Overall, regulation of de novo pyrimidine synthesis in the uracil auxotrophs of B. cepacia ATCC 25416 was observed.