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A gene‐targeting suicide vector for Streptococcus bovis
Author(s) -
Brooker J.D.,
Lum D.K.,
Thomson A.M.,
Ward H.M.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01063.x
Subject(s) - streptococcus bovis , plasmid , biology , transposable element , shuttle vector , gene , escherichia coli , vector (molecular biology) , genetics , bacteria , microbiology and biotechnology , selection (genetic algorithm) , dna , mutant , recombinant dna , rumen , food science , artificial intelligence , fermentation , computer science
J.D. BROOKER, D.K. LUM, A.M. THOMSON AND H.M. WARD. 1995. A gene‐targeting suicide vector for Streptococcus bovis has been constructed using the Escherichia coli/Streptococcus shuttle plasmid, pMU1328, and a region derived from the broad host‐range, Gram‐positive transposon, Tn916. This suicide plasmid replicates autonomously in E. coli , but not in Strep, bovis or Strep, bovis Tn916. Under positive selection, the plasmid was shown to integrate into Strep, bovis Tn916 chromosomal DNA at a frequency of 3 × 10 ‐8 cell ‐1 and was stably maintained for at least 100 generations in the absence of selection. This is the first report of a recombination system in ruminal bacteria. The ability to target genes, knock out specific functions or introduce novel genes into these micro‐organisms will allow ruminal species to be manipulated and may eventually lead to improved animal production.