Premium
Large scale cultivation of Hydrogenobacter thermophilus on hydrogen
Author(s) -
Hreggvidsson G.O.,
Gustavsson T.,
Holst O.,
Kronsbein K.,
Hjörleifsdottir S.,
Skjenstad T.,
Kristjansson J.K.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01059.x
Subject(s) - library science , political science , computer science
The thermophilic hydrogen‐oxidizing eubacterium, Hydrogenobacter thermophilus was grown with a semi‐continuous gas feed in a 150 1 fermentor, on different ratios of H 2 , O 2 (air) and CO 2 and on media of variable mineral salts composition. The best gas ratio during the exponential phase was approximately 0.5:1.0:0.03 atm of H 2 : air: CO 2 . Cell densities were increased by increasing the concentration of ammonium and phosphate in the medium. The maximum cell density obtained under the most favourable conditions was 2.62 g wet wt 1 ‐1 , corresponding to an absorption of 1.6 at 600 nm. The maximum specific growth rate ( u ) was 0.44 h ‐1 .