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Isolation and characterization of four polycyclic aromatic hydrocarbon degrading bacteria from soil near an oil refinery
Author(s) -
Ashok B.T.,
Saxena S.,
Musarrat J.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01052.x
Subject(s) - phenanthrene , alcaligenes , naphthalene , bacteria , micrococcus , anthracene , pseudomonas , hydrocarbon , creosote , polycyclic aromatic hydrocarbon , microbiology and biotechnology , aromatic hydrocarbon , chemistry , corynebacterium , isolation (microbiology) , pseudomonadaceae , biology , environmental chemistry , organic chemistry , genetics
Four bacterial strains (I‐IV) capable of optimum growth on 0·1% naphthalene, anthracene or a mixture of naphthalene and phenanthrene were isolated from soil near an oil refinery. Two isolates (I and II) were identified as belonging to the genus Micrococcus , while strains III and IV were identified as Pseudomonas and Atcaligenes respectively. All the isolates were found to bear high molecular weight plasmid DNA (isolate I and IV 89%, II 67·5% and III 92·1% of Λ DNA), which is presumed to aid in the metabolism of polycyclic aromatic hydrocarbons. The strains also showed appreciable growth at high concentrations of NaCl (up to 7·5%).