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Effects of biocides on the transduction of Pseudomonas aeruginosa PAO by F116 bacteriophage
Author(s) -
Maillard J.Y.,
Beggs T.S.,
Day M.J.,
Hudson R. A.,
Russell A.D.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01044.x
Subject(s) - welsh , library science , pharmacy , autograph , medicine , history , art history , family medicine , archaeology , computer science
The transduction of Pseudomonas aeruginosa PAO by the F116 bacteriophage was monitored after phage treatment with seven biocides commonly used in disinfection processes. Ethanol (70% and 100%), isopropanol (100%) and phenol (2%) did not affect the ability of the phage to transduce. However, chlorhexidine diacetate (1%), cetylpyridinium chloride (0·05%), glutaraldehyde (2%), peracetic acid (1%) and sodium hypochlorite (0·1%) significantly inhibited F116 transduction. The transduction process was more sensitive to biocides than the phage itself. Inactivation of F116 and inhibition of the transduction process appeared to involve two distinct mechanisms implying that the process depends on cellular target sites not affected by some biocides.