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Rapid mitochondrial probes for analysis of polymorphisms in Fusarium oxysporum special forms
Author(s) -
Bridge P.D.,
Hopkinson L.A.,
Rutherford M.A.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01040.x
Subject(s) - mitochondrial dna , fusarium oxysporum , biology , restriction fragment length polymorphism , dna , microbiology and biotechnology , cloning (programming) , molecular cloning , southern blot , genetics , polymerase chain reaction , gene , complementary dna , computer science , programming language
A method is described for the production of simple mitochondrial DNA probes from filamentous fungi for the partial characterization of mitochondrial DNA without the need for cloning, gradient centrifugation or PCR amplification. A probe (P449) consisting of a 3·38 kb mitochondrial fragment from an isolate of Fusarium oxysporum special form cubense was used to determine RFLPs in restriction digests of total DNA from 28 isolates of F. oxysporum from a variety of hosts and locations. The probe showed mtDNA polymorphisms within and between different special forms.