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Expression of lactococcin A and pediocin PA‐1 in heterologous hosts
Author(s) -
Chikindas M.L.,
Venema K.,
Ledeboer A.M.,
Venema G.,
Kok J.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01037.x
Subject(s) - heterologous expression , heterologous , biology , microbiology and biotechnology , bacteriocin , bacteria , virology , gene , recombinant dna , genetics
Pediocin PA‐1 production, immunity and secretion are specified by a cluster of four genes in Pediococcus acidilactici PAC1.0. The production by, secretion of, and immunity to lactococcin A of Lactococcus lactis are also determined by four genes. Here, expression of the pediocin operon in Lactococcus lactis is reported, which could only be achieved by placing it under control of a lactococcal promoter. Expression of the lactococcin A operon in Pediococcus is also described: recombinant clones of Pediococcus were obtained that produced and secreted both active pediocin PA‐1 and lactococcin A.