Premium
Enhanced biodegradation and emulsification of crude oil and hyperproduction of biosurfactants by a gamma ray‐induced mutant of Pseudomonas aeruginosa
Author(s) -
Iqbal S.,
Khalid Z.M.,
Malik K.A.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01035.x
Subject(s) - heptadecane , mutant , pseudomonas aeruginosa , pseudomonadaceae , chemistry , emulsion , pseudomonadales , hydrocarbon , strain (injury) , bacteria , food science , biodegradation , pseudomonas , microbiology and biotechnology , bacterial growth , energy source , biochemistry , chromatography , biology , organic chemistry , gene , petroleum , genetics , anatomy
A gamma ray‐induced mutant of Pseudomonas aeruginosa strain S8, capable of hyperproduction of biosurfactant from hydrocarbons, was isolated and named as EBN‐8. The mutant showed 3–4 times more hydrocarbon emulsification/conversion as compared to the parent when grown on Khaskheli crude oil in minimal medium. Enhanced biosurfactant production and hydrocarbon utilization by the mutant was also observed during growth on heptadecane in minimal medium as indicated by emulsion index and surface tension of cell‐free culture broth. Using heptadecane as carbon and energy source, time course for the growth (cfu ml ‐1 ) and biosurfactant production were compared for both parent and mutant. These studies were carried out for 24 d at 30 ± 2°C and for 20 d at 37°C. Growth of EBN‐8 was much faster compared to the parent as well as being 2–3 times more hyperproductive.